Eur J Obstet Gynecol Reprod Biol. 2014 Oct;181:267-74. doi: 10.1016/j.ejogrb.2014.07.037. Epub 2014 Aug 20.
Bobek V1, Kolostova K2, Kucera E3.
Author information 1Department of Laboratory Genetics, University Hospital Kralovske Vinohrady, Prague, Czech Republic; Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland; 3rd Department of Surgery, First Faculty of Medici
Eur J Obstet Gynecol Reprod Biol
AbstractOBJECTIVES: Endometriosis is a common disorder amongst women of reproductive age. Despite extensive research, no reliable blood tests currently exist for the diagnosis of endometriosisSTUDY DESIGN: We report several new approaches enabling study of cell specific characteristic of endometrial cells, introducing enrichment and culturing of viable circulating endometrial cells (CECs) isolated from peripheral blood (PB) and peritoneal endometrial cells (PECs) from peritoneal washing (PW). Size-based enrichment method (MetaCell(®), Czech Republic) has been used for the filtration of PB and PW in patients with diagnosed endometriosis.RESULTS: The PECs were found in the PW in all of the tested patients (n=17), but CECs) only in 23.5% (4/17) cases. Their endometrial origin has been proved by immunohistochemistry. PECs were successfully cultured in vitro directly on the separating membrane (9/17) exhibiting both endometrial cell phenotypes: stromal and glandular within the culture. CECs were successfully cultured in the two of the four positive cases, but in none of them confluence has been reached. The occurrence in CECs in PB is clear and very specific evidence of an active endometrial disease.CONCLUSIONS: We demonstrated efficient, quick and user friendly endometrial cells capture platform based on a cell size. Furthermore, we demonstrated an ability to culture the captured cells, a critical requirement for post-isolation cellular analysis directed to better understanding of endometriosis pathogenesis.Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.
Full Text Source: Elsevier Science
PMID:25195200 | http://www.ncbi.nlm.nih.gov/pubmed/25195200