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Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosis.

Eur J Obstet Gynecol Reprod Biol. 2014 Jan;172:85-92. doi: 10.1016/j.ejogrb.2013.10.007. Epub 2013 Oct 14.

Laudanski P1, Charkiewicz R2, Kuzmicki M3, Szamatowicz J3, Swiatecka J4, Mroczko B5, Niklinski J2.
Author information 1Department of Perinatology, Medical University of Bialystok, ul. Marii Sklodowskiej-Curie 24a, 15-276 Bialystok, Poland. Electronic address: plauda@umb.edu.pl.2Department of Clinical Molecular Biology, Medical University of Bialystok, ul. Waszyngtona 13, 15-276 Bialystok, Poland.3Department of Gynecology and Gynecological Oncology, Medical University of Bialystok, ul. Marii Sklodowskiej-Curie 24a, 15-276 Bialystok, Poland.4Department of Reproduction and Gynecological Endocrinology, Medical University of Bialystok, ul. Marii Sklodowskiej-Curie 24a, 15-276 Bialystok, Poland.5Department of Biochemical Diagnostics, Medical University of Bialystok, ul. Waszyngtona 15a, 15-276 Bialystok, Poland.
Eur J Obstet Gynecol Reprod Biol
ABSTRACT
OBJECTIVE: To compare the expression level of the most relevant angiogenesis-related genes in the eutopic endometrium of women with and without endometriosis.STUDY DESIGN: 32 regularly menstruating patients (18 with endometriosis and 14 controls) underwent surgery in the proliferative phase of the cycle. Eutopic endometrium was collected by the use of aspirating biopsy prior to laparoscopy. Only patients with advanced (stage III and IV) histopathologically confirmed ovarian endometriosis were studied. Real-time PCR gene arrays were applied to examine the expression of 84 human angiogenesis-connected genes. Western-blot and enzyme-linked immunosorbent assays (ELISA) were used to confirm the expression of selected proteins.RESULTS: We found significantly higher levels of AKT1 (p=0.003), TYMP (p=0.02), JAG1 (p=0.007), LAMA5 (p=0.005) and TIMP-1 (p=0.03) in eutopic endometrium of patients with endometriosis as compared with controls. By the use of Western blot we found clearly positive expression of AKT1 whereas ELISA assays confirmed expression of AKT1, TYMP, JAG1, LAMA5 and TIMP1.CONCLUSION: Changes in the expression of selected genes might lead to or be a consequence of an early defect in the physiological activity of proliferative endometrium ultimately resulting in its overgrowth outside the uterine cavity.Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Full Text Source: Elsevier Science
PMID:24188612 | http://www.ncbi.nlm.nih.gov/pubmed/24188612

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